Regulatory

Part:BBa_K190023:Design

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-08-25)
Revision as of 18:36, 20 October 2009 by Nienke (Talk | contribs) (→‎Source)

Arsenic Promoter (ArsR regulated) with own RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The arsR promoter is present in most E. coli strains, and is regulated by ArsR dimer complex. Using the sequence of the promoter in our own E. coli TOP10 cells, oligo's were designed and annealed to produce the promoter with EcoRI and SpeI (prefix and suffix) overhangs.

References