Generator

Part:BBa_K265011

Designed by: Richard Hauser   Group: iGEM09_UC_Davis   (2009-10-15)
Revision as of 18:34, 20 October 2009 by TimFenton (Talk | contribs)

OmpA with proteolytic cleavage tag

This part is a fusion of a modified OmpA with a signal sequence to allow cleavage when a protein is fused. OmpA was modified by replacing the normal restriction sites with sites for RFC12 fusion format. An OmpA/protein fusion normally allows display of proteins on the cell surface by way of a linker. The addition of this signal sequence allows secretion by cleavage from the OmpA linker after display.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aOmpA with proteolytic cleavage tag