Generator
Part:BBa_K265011
Designed by: Richard Hauser Group: iGEM09_UC_Davis (2009-10-15)
OmpA with proteolytic cleavage tag
This part is a fusion of a modified OmpA with a signal sequence to allow cleavage when a protein is fused. OmpA was modified by replacing the normal restriction sites with sites for RFC12 fusion format. An OmpA/protein fusion normally allows display of proteins on the cell surface by way of a linker. The addition of this signal sequence allows secretion by cleavage from the OmpA linker after display.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
n/a | OmpA with proteolytic cleavage tag |