Coding

Part:BBa_K4821005

Designed by: Madeline Ho   Group: iGEM23_Washington   (2023-10-12)
Revision as of 10:15, 12 October 2023 by Registry (Talk | contribs)

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RdhANp_M132O_K12

This sequence codes for a modified RdhANp that has been docked to the ortho chlorine position in PCB 132 and codon-optimized for expression in E. coli K-12. It was designed as an improvement on part BBa_K4821002, the codon-optimized wild-type RdhANp dehalogenase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1628
    Illegal BamHI site found at 312
    Illegal BamHI site found at 419
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None