Regulatory

Part:BBa_K4770024

Designed by: Andrea Camí Bonet   Group: iGEM23_Barcelona-UB   (2023-10-12)
Revision as of 10:11, 12 October 2023 by Andreacami (Talk | contribs) (→‎Description and Biology)


PNIT_Nitrate_Dependent_Promoter


PNIT_Nitrate_Dependent_Promoter

Description and Biology

PNIT is a nitrate-dependent promoter highly induced by nitrates and repressed by ammonia in Chlamydomonas reinhardtii. It activates the transcription of nitrate assimilation genes such as NR or NiR under nitrate-stress conditions. Contrary, it is repressed upon ammonium, which is the product resulting from said nitrate assimilation.

How can this part be used

In AlgaGenix, PNIT sequence has been adapted for MoClo assembly as a A1-B2 part.

Source of this part

Original sequence was obtained from MoClo kit designed by Crozet, et. al (2018)

Design considerations

MoClo kit provides you with sequences with different overhangs so you can choose the one that better fits you design/cloning plan.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 197
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 197
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None