RBS

Part:BBa_K4814012

Designed by: CHIEN-YUEH LIU   Group: iGEM23_PuiChing-Macau   (2023-09-28)
Revision as of 09:54, 12 October 2023 by Ginaliu (Talk | contribs)


Strong RBS

To attempt to improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates. Sequence: TTTAAGAGGGGGCTATACAT

Click here for the experiment data: RecA(K3)-Strong-eGFP BBa_K4814013

References:

Zhang, M., Holowko, M. B., Zumpe, H. H., & Ong, C. S. (2022). Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site. ACS Synthetic Biology, 11(7), 2314-2326. https://doi.org/10.1021/acssynbio.2c00015

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None