Part:BBa_K4825044:Design

ERG20ww-t48PS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 222
  Illegal BglII site found at 1974
  Illegal BamHI site found at 1059
  Illegal BamHI site found at 2811
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 694
  Illegal BsaI.rc site found at 2446
  

Design Notes

In our engineering design, we overexpressed ERG20ww in S. cerevisiae, in which the 96th amino acid, F, and the 127th amino acid, N of the part were both mutated to W amino acid in order to decrease the formation of FPP while maintaining its function to increase the supply of GPP. t48PS encoding for alpha-pinene synthase is a heterologous gene which is originally from Pinus taeda. It is responsible for the conversion from GPP to alpha-pinene. To increase the production, the 48 amino acids at the N-terminus of the part is truncated.

Source

Saccharomyces cerevisiae and Pinus taeda

References

CHEN Tianhua, ZHANG Ruosi, JIANG Guozhen, YAO Mingdong, LIU Hong, WANG Ying, XIAO Wenhai, YUAN Yingjin. Metabolic engineering of Saccharomyces cerevisiae for pinene production[J]. CIESC Journal, 2019, 70(1): 179-188