Coding

Part:BBa_K4645008

Designed by: Meng Sun   Group: iGEM23_HZAU-China   (2023-10-10)
Revision as of 08:46, 12 October 2023 by Felicitysm (Talk | contribs)

A chimeric lysozyme with secretory ability.

ClyR is a chimeric lysin, comprising a cysteine, histidine-dependent amidohydrolase/peptidase catalytic domain from PlyC lysin, and the cell-wall binding domain from PlySs2 lysin. The signal peptide OmpA enables ClyR to be secreted into the periplasmic space of bacteria.


Result

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Figure 1. Colony PCR assay of the OmpA-ClyR fragment.

We used homologous recombination in which the OmpA signal peptide was added to the N-terminus of ClyR and transformed into E.coli DH5α competent cells and incubated overnight at 37℃. Below is a plot of colony PCR results.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 816
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 184
    Illegal AgeI site found at 268
    Illegal AgeI site found at 583
    Illegal AgeI site found at 766
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None