Coding

Part:BBa_K4837000

Designed by: Tse Cheong Tat   Group: iGEM23_HK-SKHLPSS   (2023-10-10)
Revision as of 08:41, 12 October 2023 by Lpctt (Talk | contribs)

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Lignin peroxidase

Optimized the sequence for E. coli expression

This plasmid optimized the sequence from white-rot fungus ( Phanerochaete chrysosporium) for E. coli expression.


The product illustrated below, namely lignin peroxidase (LiP), works together with a DNA duplex and another product from BBa_K4837001 to form a "cascade enzyme system" for PFAs degradation.

LiP is conjugated with an azide-modified DNA oligo, which is illustrated below:

Finally, the "cascade enzyme system" formed when two DNA oligos in the conjugated products bound with each other by complementary base pairing principle, which is illustrated below:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 126
    Illegal NheI site found at 1694
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 66
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 260
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2352


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