Coding

Part:BBa_K4645008

Designed by: Meng Sun   Group: iGEM23_HZAU-China   (2023-10-10)
Revision as of 08:40, 12 October 2023 by Felicitysm (Talk | contribs)


A chimeric lysozyme with secretory ability.

ClyR is a chimeric lysin, comprising a cysteine, histidine-dependent amidohydrolase/peptidase catalytic domain from PlyC lysin, and the cell-wall binding domain from PlySs2 lysin. The signal peptide OmpA enables ClyR to be secreted into the periplasmic space of bacteria.


Result

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Figure 1. Colony PCR assay of the OmpA-ClyR fragment.

We used homologous recombination in which the OmpA signal peptide was added to the N-terminus of ClyR and transformed into E.coli DH5α competent cells and incubated overnight at 37℃. Below is a plot of colony PCR results.

Sequence and Features BBa_K4645008 SequenceAndFeatures

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