Composite

Part:BBa_K4604022

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-08)
Revision as of 07:56, 12 October 2023 by NikitaEdel (Talk | contribs)


piG_10b (tetR_bluB_T7RBS_riboK12_mazF) Plasmid piG_10 is made up of the tet promoter/repressor, bluB, a riboswitch for AdoCbl, the tet RBS, mazF (toxin) and the rrnB terminator. The backbone we utilized was pGGAselect, a plasmid commonly used with golden gate cloning that has a chloramphenicol resistance. This BioBrick is BBa_K4604021 with an additional RBS.

Notice

We have performed western blot after induction of this BioBrick, however we could not detect any BluB expression. The reason for this is not known to us, however for the sake of completion and to allow further exploration, we wanted to upload this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1603
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2252


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Categories
Parameters
None