Composite

Part:BBa_K4724028:Design

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-10)
Revision as of 06:37, 12 October 2023 by Moxi977 (Talk | contribs) (→‎Source)

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IsPETaseQ119F/W159H-Linker-CBM11-6xHis Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
    Illegal PstI site found at 913
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
    Illegal PstI site found at 913
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1134
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
    Illegal PstI site found at 913
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal PstI site found at 913
    Illegal AgeI site found at 546
    Illegal AgeI site found at 926
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We needed to purify the IsPETase protein, so we attached the 6xHis tag after IsPETase.

Source

This is a new section that is followed by a domain in the IsPETase double mutant.

References