Plasmid

Part:BBa_K4815015:Design

Designed by: Chen Xi   Group: iGEM23_NJU-China   (2023-10-12)
Revision as of 06:32, 12 October 2023 by Registry (Talk | contribs)

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pDualPH5-pDual mcherry-yeGFP High 5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 3641
    Illegal PstI site found at 964
    Illegal PstI site found at 2196
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 396
    Illegal NheI site found at 3407
    Illegal PstI site found at 964
    Illegal PstI site found at 2196
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 396
    Illegal BglII site found at 1927
    Illegal BamHI site found at 3604
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 3641
    Illegal PstI site found at 964
    Illegal PstI site found at 2196
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 3641
    Illegal PstI site found at 964
    Illegal PstI site found at 2196
    Illegal NgoMIV site found at 1826
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2886
    Illegal BsaI.rc site found at 4281
    Illegal SapI site found at 4772
    Illegal SapI site found at 5372
    Illegal SapI.rc site found at 2733


Design Notes

1


Source

1

References