RNA

Part:BBa_K4664001

Designed by: Yiming Dong   Group: iGEM23_HS-China   (2023-10-09)
Revision as of 06:03, 12 October 2023 by Chrisdong (Talk | contribs)

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miRNA for COPD

MiR-1274a regulates the expression of Forkhead box O (FOXO) 4, which links to oxidative stress or cellular aging. It is related to the presence of COPD as well as cellular senescence in COPD.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Background

miR-1274a is responsible for the regulation of the FOXO4 gene, which in turn regulates SOD2 and SIRT1, the dysfunction of the latter found commonly not only in COPD patients but in long-term cigarette smokers also. Research has also discovered that reduced expression of SIRT1 may be found in the peripheral lung tissues and serum of COPD patients.
miR-1274a is also significantly upregulated in COPD patients.
RT-qPCR (Reverse Transcription Quantitative Real-time Polymerase Chain Reaction), is a combination of RT-PCR and qPCR methods, and is commonly employed for the detection and quantification of RNA. The procedure involves the enzyme reverse transcriptase converting total RNA or messenger RNA (mRNA) to complementary DNA (cDNA). This cDNA is then amplified and used in quantitative PCR (qPCR) or real-time PCR to detect specific targets. This PCR method utilises a number of fluorescent chemicals to quantify the amount of DNA at each cycle in real-time.

Design

Sequence:
GUCCCUGUUCAGGCGCCA

Results

The following charts show the RT-qPCR results for miR-1274a.
p464001-1.png Figure 1. Results for miR-1274a RT-qPCR.

References

Roffel, M. P., Maes, T., Brandsma, C., Van Den Berge, M., Vanaudenaerde, B. M., Joos, G., Brusselle, G., Heijink, I. H., & Bracke, K. (2021). MiR-223 is increased in lungs of patients with COPD and modulates cigarette smoke-induced pulmonary inflammation. American Journal of Physiology-lung Cellular and Molecular Physiology, 321(6), L1091–L1104. https://doi.org/10.1152/ajplung.00252.2021

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