Regulatory
Part:BBa_K4634018
Designed by: Ng Swee Hong Group: iGEM23_Tsinghua (2023-10-10)
Revision as of 05:23, 12 October 2023 by NgSweeHong (Talk | contribs)
Temperature-sensitive Promoter: pBV220_PL-2, modified from PL promoter.
Here we hope to optimize PL promoter, enabling a higher expression level at 42 °C. The following content mainly describes the pBV220_PL2 which shows prominent improvement during our experiment. Considering the completeness of the project, the nuclear acid sequence as well as corresponding experimental data of other promoters are also listed below.
Characterization
Introduction
PL and PR promoter, which are ubiquitously employed in bacterial expression systems, are typical promoters regulated by temperature and . In pBV220, they are located tandemly. The repressor, Tcl857, is a temperature sensitive mutant of phage λ. Under normal condition, Tcl857 act as dimers that bind to the OR1, OR2 and OR3 operator site, hindering the occurrence of transcription. When the temperature rises to about 42 °C, the repressor is thus denatured and the transcription happens.[1]Usage and biology
Compared to PL wild type, the Temperature-sensitive promoter X shows a higher promoter activity after heat treatment. Sequence and Features
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Categories
Parameters
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