Regulatory

Part:BBa_K239006

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-26)
Revision as of 15:44, 20 October 2009 by Axelnystrom (Talk | contribs)

Modified NarK promoter

Sequence contains 2 Fnr (one of which is modified), 1 Fis, 2 NarL binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic metabolism. It is designed to have better affinity to activated Fnr and hence stronger activity under anaerobic conditions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aModified NarK promoter