Regulatory

Part:BBa_K239005

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-26)
Revision as of 15:41, 20 October 2009 by Axelnystrom (Talk | contribs)

NarK promoter, Fnr activated under anaerobic conditions

Sequence contains 2 Fnr, 1 Fis, 4 NarL and 1 IHF binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions.

Area of application: Detection of when the cell is switching to anaerobic metabolism.

Experiments by Kolesnikow et al. (1992) has showed that NarK expression is upregulated 100-fold under anaerobic condtitions and a further 8-fold in the presence of nitrate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/control
Parameters
None