Generator

Part:BBa_K283016

Designed by: W Ling , Nelson Chu, Shi Lei   Group: iGEM09_HKU-HKBU   (2009-10-13)
Revision as of 15:32, 20 October 2009 by NelsonChu (Talk | contribs)

pLacI-rbs-cheZ_histag-terminators

The pLac can be repressed by LacI protein which can be inhibited by IPTG. The cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement and is tagged by 6-Histidine tail. At the beginnning, we intend to use this part to control the speed of our Bactormotor by adjusting the concentration of inducer IPTG. However, we found the leaky expression is too high. Then our group combined it with to construct a new biobrick BBa_K283014. Unfortunately, we found that it still high. In the end, we created speed control device BBa_K283002 instead of it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 872
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/generator/prct
//function/motility
Parameters
None