Regulatory

Part:BBa_K4600002:Experience

Designed by: Kavirang Biswas   Group: iGEM23_Warwick   (2023-10-08)
Revision as of 02:38, 12 October 2023 by KavirangB (Talk | contribs) (→‎Applications of BBa_K4600002)


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Applications of BBa_K4600002

Team
Figure 1: Colony PCR where a pJBEI-6411 plasmid harbouring the TetR and AcrB pump sequence, were tested for uptake. Used a 1kb plus DNA ladder.
We designed primers that bind on either side of the TetR sequence at a distance of 709 base pairs. Only plasmids with the insert should get be cloned in the PCR. The successful colony PCR suggests that there was a successful uptake of the part by the host.

User Reviews

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