Regulatory

Part:BBa_K4788000

Designed by: Zheng Huang   Group: iGEM23_LZU-CHINA   (2023-10-05)
Revision as of 01:24, 12 October 2023 by Zcli lzu (Talk | contribs) (→‎Usage and Biology)


FadBA promoter

This is a promoter that responds to the inhibitory effect of FadR by adding the Fad O operator afterwards.

Usage and Biology

It is a promoter that can combine with the FadO operator and FadR gene to form an oleic acid inducer.For specific information on oleic acid inducers, please refer to BBa_K4788002

After forming composite components

The FadR protein expressed by the FadR gene can bind to the operator FadO following the FadBA promoter, which inhibits the initiation of FadBA. However, oleoyl CoA metabolized from oleic acid can relieve this inhibition and promote the expression of FadBA promoter. This allows it to be induced by oleic acid for expression. In practical applications, we can change the number and position of FadO operators to change their response threshold to oleic acid to achieve more precise regulatory effects.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None