DNA

Part:BBa_K4630110

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-10)
Revision as of 00:52, 12 October 2023 by James Qin (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


stgRNA-cassette 1

StgRNA-cassette 1 is the cassette form of self-targeting guide RNA 1 (stgRNA 1). It is the building block of the cascade recording system. It contains promoter, self-targeting guide RNA (stgRNA 1), double terminator.

Usage and Biology

In the cassette recorder, we integrate the two methods into a single cassette, with unique barcode (fig 1). Once triggered, double strand break (DSB) is introduced into the working plasmid, enabling the intra-plasmid recombination. It is worth noting that the two homologous arms flank the stgRNA, so the entire cassette will be deleted after the recombination, and the next cassette is expected to be brought to the proper expressing place. The inducer triggers over and over, and recorder records it by keeping changing the cassette.

Figure 1. Working pattern of the change of cassette

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None