Composite
Part:BBa_K4880021:Design
Designed by: Xinran Xia Group: iGEM23_BASIS-China (2023-09-08)
PpsbA2-sepA2-PisiA6803-sepT2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 684
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 684
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 742
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 684
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 684
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 996
Design Notes
-
Source
Zhou, Y., Sun, T., Chen, Z., Song, X., Chen, L., & Zhang, W. (2019). Development of a new biocontainment strategy in model cyanobacterium synechococcus strains. ACS Synthetic Biology, 8(11), 2576–2584. https://doi.org/10.1021/acssynbio.9b00282