Part:BBa_K4630114

stgRNA-cassette (1+2+3)

StgRNA-cassette (1+2) is the ligated form of stgRNA-cassette (1+2) and stgRNA-cassette 3. It construct the multi-level recorder allowing the biological events to trigger recording three times.

Usage and Biology

In the cassette recorder, we integrate the two methods into a single cassette, with unique barcode (fig B2). Once triggered, double strand break (DSB) is introduced into the working plasmid, enabling the intra-plasmid recombination. It is worth noting that the two homologous arms flank the stgRNA, so the entire cassette will be deleted after the recombination, and the next cassette is expected to be brought to the proper expressing place. The inducer triggers over and over, and recorder records it by keeping changing the cassette.

Figure B2. Working pattern of the change of cassette

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]