Part:BBa_K4788002

Oleic acid inducer

This is a promoter module that can respond to oleic acid induction. And it has different response intensities for different oleic acid concentrations.

Usage and Biology

The FadR protein expressed by the FadR gene can bind to the operator FadO following the FadBA promoter, which inhibits the initiation of FadBA. However, oleoyl CoA metabolized from oleic acid can relieve this inhibition and promote the expression of FadBA promoter. This allows it to be induced by oleic acid for expression. In practical applications, we can change the number and position of FadO operators to change their response threshold to oleic acid to achieve more precise regulatory effects.

Characterization

In order to characterize the oleic acid inducer, we added mRFP after the oleic acid inducer for characterization, and we validated the response of the oleic acid inducer to gradient oleic acid by inducing the initiation of the oleic acid promoter under both aerobic and anaerobic conditions, as well as the operability of the part under anaerobic conditions. For detailed information, please refer to BBa_K4788008

Reference

Ma Y, Zheng X, Lin Y, Zhang L, Yuan Y, Wang H, Winterburn J, Wu F, Wu Q, Ye JW, Chen GQ. Engineering an oleic acid-induced system for Halomonas, E. coli and Pseudomonas. Metab Eng. 2022 Jul;72:325-336. doi: 10.1016/j.ymben.2022.04.003. Epub 2022 May 2. PMID: 35513297.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

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• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]