Composite

Part:BBa_K4604021

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-07)
Revision as of 22:46, 11 October 2023 by HannahSwientek (Talk | contribs)


piG_10a (tetR_bluB_riboK12_mazF)

BioBrick piG_10a consists of the tet promoter/repressor, the modified T7 RBS, bluB, a riboswitch for AdoCbl, mazF and the rrnB terminator. The backbone we used in the experiments is pGGAselect. The tet promoter is a BioBrick from iGEM Freiburg 2022 (BBa_K4229059).

Characterization

This BioBrick is the closest we got to testing all the parts our system consists of combined. Read more about the full setup on Ba_K4604023.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1603
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2238


[edit]
Categories
Parameters
None