Plasmid
pEX

Part:BBa_K243033:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-20)
Revision as of 10:44, 20 October 2009 by Registry (Talk | contribs)

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pEX


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2734
    Illegal XbaI site found at 1523
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2735
    Illegal PstI site found at 2749
    Illegal NotI site found at 2742
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1808
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2735
    Illegal XbaI site found at 1523
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal suffix found in sequence at 2725
    Illegal XbaI site found at 1523
    Illegal NgoMIV site found at 1531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3800
    Illegal SapI.rc site found at 4799


Design Notes

Noine.


Source

By adding additional restriction sites in pMAL we create our expression vector.

References