Composite

Part:BBa_K4757062:Design

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-10-11)
Revision as of 22:31, 11 October 2023 by Registry (Talk | contribs)

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TPA sensing XylS-K38R-L224Q/Pm expressing mKate2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 212
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 934
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ps1/Ps2 tf only works with co-induction of MBA andTPA


Source

XylS/Pm is natively found in P.putida and was first discovered on the TOL Plasmid.

References