Coding
Part:BBa_K4825047:Design
Designed by: Ni Weizhao Group: iGEM23_GreatBay-SCIE (2023-10-11)
CDC9-LEU1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1163
Illegal EcoRI site found at 1627
Illegal EcoRI site found at 2917
Illegal EcoRI site found at 3706
Illegal XbaI site found at 1404
Illegal PstI site found at 2093
Illegal PstI site found at 2891
Illegal PstI site found at 3386
Illegal PstI site found at 3671 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1163
Illegal EcoRI site found at 1627
Illegal EcoRI site found at 2917
Illegal EcoRI site found at 3706
Illegal PstI site found at 2093
Illegal PstI site found at 2891
Illegal PstI site found at 3386
Illegal PstI site found at 3671 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1163
Illegal EcoRI site found at 1627
Illegal EcoRI site found at 2917
Illegal EcoRI site found at 3706 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1163
Illegal EcoRI site found at 1627
Illegal EcoRI site found at 2917
Illegal EcoRI site found at 3706
Illegal XbaI site found at 1404
Illegal PstI site found at 2093
Illegal PstI site found at 2891
Illegal PstI site found at 3386
Illegal PstI site found at 3671 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1163
Illegal EcoRI site found at 1627
Illegal EcoRI site found at 2917
Illegal EcoRI site found at 3706
Illegal XbaI site found at 1404
Illegal PstI site found at 2093
Illegal PstI site found at 2891
Illegal PstI site found at 3386
Illegal PstI site found at 3671
Illegal NgoMIV site found at 2434
Illegal AgeI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 3896
Design Notes
The composite part appends the first 47 amino acids of CDC9 to the N-terminus of LEU1 in order to relocate and overexpress LEU1 in mitochondria.
Source
Saccharomyces cerevisiae