Coding

Part:BBa_K4825046:Design

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 22:26, 11 October 2023 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


ARO10-ADH7-ATF1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal NheI site found at 2385
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal BglII site found at 3105
    Illegal BamHI site found at 2146
    Illegal BamHI site found at 4432
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 3418
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3532
    Illegal SapI.rc site found at 4540


Design Notes

By overexpression of ARO10 and ADH7, the supply of precursors isopenaldehyde and isopentanol is increased, and the overexpression of ATF1 directly enhances the corresponding conversion to isoamyl acetate. Therefore, The composite part increases the production of isoamyl acetate in S. cerevisiae.


Source

Saccharomyces cerevisiae

References