Coding
Part:BBa_K4825045
Designed by: Ni Weizhao Group: iGEM23_GreatBay-SCIE (2023-10-11)
Delete
This composite part contains the part ARO10 BBa_K4825015, ADH7 BBa_K4825016 and ATF1 BBa_K4825017. In the pathway of producing isoamyl acetate in S. cerevisiae, ARO10 encodes for 2-oxoacid decarboxylase, which is reponsible for converting 2-ketoisocaproate (KIC) to isopentaldehyde. ADH7, which encodes for alcohol dehydrogenase 7, is responsible for converting isopentaldehyde to isopantanol. ATF1 encodes for alcohol acetyltransferase, which catalyzes the conversion of isopenanol to isopentyl acetate (isoamyl acetate).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 222
Illegal BglII site found at 1974
Illegal BamHI site found at 1059
Illegal BamHI site found at 2811 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 694
Illegal BsaI.rc site found at 2446
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Categories
Parameters
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