Regulatory

Part:BBa_K4788002:Design

Designed by: Zheng Huang   Group: iGEM23_LZU-CHINA   (2023-10-05)
Revision as of 21:52, 11 October 2023 by Zcli lzu (Talk | contribs) (→‎Design Notes)


Oleic acid inducer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The front of this part requires a promoter to express the FadR protein in order to achieve oleic acid response function. Therefore, in practical use, appropriate promoters or plasmid skeletons should be selected. In longer-term applications, we can try to change the number and position of FadO operators to change the oleic acid induction threshold to achieve more precise promoter regulation.

Source

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References