Coding
Part:BBa_K4830015
Designed by: Beibitkyzy Aruzhan Group: iGEM23_NTU-Singapore (2023-10-10)
Revision as of 21:06, 11 October 2023 by Aruzhan001 (Talk | contribs)
PFV RT
Prototype Foamy virus Reverse transcriptase.
Usage and Biology
PFV RT retrieved from chimpanzee was human codon optimized and fused to nickase Cas9 to form prime editor.
Characterization
It was tested on endogeneous sites as well TLR reporter using HEK293T cells to evaluate the alternative RT performance in terms of its ability to introduce different edits on different target sites.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/eukaryote/human
biology | Eastern chimpanzee simian foamy virus |
protein | Reverse Transcriptase |