Protein_Domain

Part:BBa_K203101

Designed by: Michael Bartoschek and Douaa Mugahid   Group: iGEM09_Heidelberg   (2009-10-15)
Revision as of 08:45, 20 October 2009 by Larsplus (Talk | contribs) (→‎Functional Parameters)

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ER membrane targeting (C-terminal)

This part is a protein domain of the Sar-1 GTPase known to localize to the ER membrane. It is very convenient as a localization signal to the ER for proteins to which it is fused.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 133
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 133
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

n/aER membrane targeting (C-terminal)

Shown to localize proteins to the ER. An expression device consisting of Part:BBa_I712004 (CMV) Part:BBa_K203103 (GFP), Part:BBa_K203101 (Sar-1) and Part:BBa_K203102 (terminator) was assembled for this characterization.

ER2.jpg

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Categories
Parameters
n/aER membrane targeting (C-terminal)