Regulatory

Part:BBa_K203107

Designed by: Corinna Hiller and Hannah Uckelmann   Group: iGEM09_Heidelberg   (2009-10-15)
Revision as of 07:23, 20 October 2009 by Chiller (Talk | contribs) (→‎Functional Parameters)

c-Jun promoter (growth factor regulated)

This part is a natural c-Jun promoter and when placed upstream of other genes could induce their transcription in response to Epidermal Growth Factor to the medium.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 398
    Illegal BamHI site found at 392
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 110


Functional Parameters

n/ac-Jun promoter (growth factor regulated)

The natural c-Jun promoter was integrated into Part:BBa_K203100 and was induced by Epidermal Growth Factor (diluted 1:20000 in DMEM++) in HeLa cells. The GFP expression level was determined in REU by flow cytometry (1 experiment, 3 replicates) 3 hours after induction.

BBa_K203107 is a natural c-Jun promoter induced more than 100% by Epidermal Growth Factor. The c-Jun promoter/Part:BBa_K203100 construct is coupled to GFP and induced with Epidermal Growth Factor (diluted 1:20000 in DMEM++) for three hours. The GFP fluorescence of the c-Jun promoter (in REU) was measured by flow cytometry once (à three replicates).The standard deviation is indicated by the error bars.


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Categories
Parameters
n/ac-Jun promoter (growth factor regulated)