Part:BBa_K2549044
CD8alpha signal peptide
CD8α signal peptide guides synthesized fusion protein to pass the translocon[1] into the endoplasmic reticulum[2], and the fusion protein will be later sugar modified in Golgi[3], presented on the plasma membrane and located to the outside of the cell. We include this part as it is required for the parts collection Part:BBa_K2549016 ~ Part:BBa_K2549043.
Literature Characterization by AFCM-Egypt
The study created a reporter construct by joining the C-terminus of CD63, one of the most used exosome markers, to nanoluc (nluc), a tiny and potent bioluminescence reporter10. After progressive centrifugation to eliminate masking signals12, luminescence in the cell-culture supernatant was measured. This reporter gene was co-transfected with plasmids expressing potential candidates for exosome production augmentation.
Poly(A) RNA samples from each cell line were separated by gel electrophoresis, transferred to a nitrocellulose membrane, and hybridized with 32P-labeled cDNA probes specific for MC-CPA or CD8amp;. The membranes were then reprobed with a 32P-labeled p-actin cDNA probe to verify that equal amounts of RNA had been loaded in each lane. The results showed that MC-CPA mRNA was expressed at a higher level in MW9.11-3 cells than in MC/9.IL-4 cells, while CDalpha mRNA was expressed at a similar level in both cell lines. These findings suggest that MC-CPA and CDalpha may play different roles in the development and differentiation of these two cell
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
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