Composite

Part:BBa_K4645016

Designed by: Yutong Chen   Group: iGEM23_HZAU-China   (2023-10-11)
Revision as of 18:40, 11 October 2023 by Lloheha (Talk | contribs)


J23100-B0030-vqmA-B0017-Pqtip-tetR-B0015-PTet-amcyan-B0017

Verification of Not-Gate.


Usage and Biology

We transformed this biobrick into E. coli to regulate expression of genes. With the growing of bacteria, DPO accumulates. While the concentration of DPO reached the threshold value, the expression of NeuA, NeuB and TetR would be activated. Then TetR shut down the expression of amcyan in downstream.

What？


Figure 1. The circuit we assembled.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 599
    Illegal AgeI site found at 2547
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None