Coding

Part:BBa_K4879004:Design

Designed by: Apoorva Gopal   Group: iGEM23_IISER-Pune-India   (2023-10-08)
Revision as of 18:28, 11 October 2023 by Sauron 3791 (Talk | contribs)

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Yarrowia lipolytica guaB marker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1019
    Illegal PstI site found at 878
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1019
    Illegal PstI site found at 878
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1019
    Illegal BamHI site found at 1081
    Illegal XhoI site found at 851
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1019
    Illegal PstI site found at 878
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1019
    Illegal PstI site found at 878
    Illegal NgoMIV site found at 868
    Illegal NgoMIV site found at 1093
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence has been codon-optimized for Y. lipolytica using SnapGene. It also has silent mutations to make the part Type II Standard compatible.


Source

The part had been synthesized by IDT, iGEM's DNA synthesis partner.


References

Wagner, J.M., Williams, E.V. and Alper, H.S. (2018), Developing a piggyBac Transposon System and Compatible Selection Markers for Insertional Mutagenesis and Genome Engineering in Yarrowia lipolytica. Biotechnol. J., 13: 1800022. https://doi.org/10.1002/biot.201800022