Generator
PL luxI

Part:BBa_K193001

Designed by: Shinya Tahara   Group: iGEM09_Tokyo_Tech   (2009-10-14)
Revision as of 05:55, 20 October 2009 by Shinya (Talk | contribs)

CI repressible LuxI enzyme generator

A cI repressible promoter driving production LuxI enzyme which produces an AHL (3OC6HSL).

This part is used to transform E.coli strain N99,which express cI constitutively, and N4830,which express temperature-sensitive cI constitutively.

We improve existing BioBrick Part F1610(https://parts.igem.org/Part:BBa_F1610), and construct K193001.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 712
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None