Coding

Part:BBa_K4830028:Design

Designed by: Ong Jie Hao   Group: iGEM23_NTU-Singapore   (2023-10-11)
Revision as of 17:33, 11 October 2023 by Jong154 (Talk | contribs) (→‎References)


eGFP_simple premature stop codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A premature stop codon has to be installed into a functional eGFP.


Source

The eGFP is derived from Aequorea victoria. It has an excitation maxima of 488nm, and emission maxima of 509nm.


References

Certo, M. T., Ryu, B. Y., Annis, J. E., Garibov, M., Jarjour, J., Rawlings, D. J., & Scharenberg, A. M. (2011). Tracking genome engineering outcome at individual DNA breakpoints. Nature Methods, 8(8), 671–676.