Part:BBa_K228867

pSB1A2-lacZ screening plasmid

This part is an improved version of pSB1A2, which lacks the NotI in between SpeI and PstI site. A constitutive lacZ alpha generator is placed in the original NotI site. Usually, rbs parts and promoter parts which is relatively small and has a low production after gel purification can be placed in this backbone. This improved plasmid can support blue white screening. Because the original parts on this plasmid is small parts, it is digested to be a vector. By cutting the plasmid with SpeI and PstI, the lacZ generator is removed. Although false positive can be created by religation of singal digestion product, it can be distinguished from the correct colonies by its color. Correct colonies are white while false positive ones are blue.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a suffix.
  Illegal SpeI site found at 2
  Illegal PstI site found at 303
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2345
  Illegal NheI site found at 14
  Illegal NheI site found at 37
  Illegal SpeI site found at 2
  Illegal PstI site found at 303
  Illegal NotI site found at 2351
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2345
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2345
  Plasmid lacks a suffix.
  Illegal SpeI site found at 2
  Illegal PstI site found at 303
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2345
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2360
  Illegal SpeI site found at 2
  Illegal PstI site found at 303
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1384