Coding

Part:BBa_K4825023

Designed by: Ni Weizhao   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 15:45, 11 October 2023 by Registry (Talk | contribs)

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pGAL1

"pGAL1 is a strong inducible promoter of GAL gene family, functioned as a transcriptional factor that is regulated by the level of galactose and repressed by the presence of glucose.

The galactose inductibility of this part has been widely used in heterologous protein expression. In our project, this part is utilized to construct a plasmid with Bax gene to fulfill the kill switch design with a logic gate induced by galactose in S.cerevisiae. "

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None