RNA

Part:BBa_K4830023

Designed by: Ong Jie Hao   Group: iGEM23_NTU-Singapore   (2023-10-11)
Revision as of 15:37, 11 October 2023 by Registry (Talk | contribs)

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PETLR pegRNA 1

PETLR pegRNA 1 - prime editing guide RNA (pegRNA) targeting the premature stop codon instilled in an eGFP; The premature stop codon is edited into the translatable amino acid - threonine, restoring the wild-type eGFP sequence. The PETLR pegRNA 1 is used in the improved Traffic Light Reporter (TLR) assay for prime editing adapted from the TLR paper in the References.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/gfp
//classic/reporter
Parameters
biologyHuman
colorGreen