RNA
Part:BBa_K4830022:Design
Designed by: Ong Jie Hao Group: iGEM23_NTU-Singapore (2023-10-11)
TLR ngRNA 2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No design considerations.
Source
The TLR ngRNA 2 design contains the spacer sequence, which was adapted from other ngRNAs in the Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized those and other necessary sequences, and further combined with a 65777 backbone with a mCherry-stop-Clover insertion in our lab.