Part:BBa_K4645004

VqmAphage

This part is meant to express vqmA gene which would translate into protein VqmA_Phage.
Vibrio cholerae uses a quorum-sensing (QS) system composed of the autoinducer 3,5-dimethylpyrazin-2-ol (DPO) and receptor VqmA, which together repress genes for virulence and biofilm formation. VqmA_Vc activates expression of the vqmR gene encoding the small regulatory RNA VqmR. Vibriophage VP882 encodes a VqmA homolog called VqmA_Phage that activates the qtip promoter (Pqtip). It’s worth noting that, VqmA_Phage can activate vqmR expression but VqmA_Vc cannot activate expression of qtip. Promoter selectivity is driven by each VqmA DNA-binding domain and key DNA sequences in the vqmR and qtip promoters are required to maintain specificity.
Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 535
  
• 1000
  COMPATIBLE WITH RFC[1000]