Coding
Cuting Sil

Part:BBa_K4623007:Design

Designed by: Ruixiao Tan   Group: iGEM23_BNU-China   (2023-10-09)
Revision as of 15:18, 11 October 2023 by Dooooog (Talk | contribs) (→‎References)

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Cut Silinker 2, cutsite-linker, GP41-1-PLGVR-NrdJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence includes the stop codon,The PLGVR sequence can be replaced with any desired cleavable peptide.


Source

This part sequence is primarily derived from an artificially modified amino acid sequence that has been optimized based on E. coli codons.

References

[1] Lun Yang, Jian Tang, Hui Yin, Jie Yang, Bin Xu, Yunkun Liu, Zhi Hu, Bentong Yu, Fangfang Xia, and Guowen Zou ACS Biomaterials Science & Engineering 2022 8 (2), 880-892 DOI: 10.1021/acsbiomaterials.1c01418.