Composite

Part:BBa_K4642059

Designed by: Ryan Yapa   Group: iGEM23_City-of-London-UK   (2023-10-10)
Revision as of 14:58, 11 October 2023 by RishabhDas (Talk | contribs)

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T7 / Breast Cancer / Endometriosis / Ovarian Cancer / PCOS / Double Terminator

Our switches, BBa_K4642021, BBa_K4642005, BBa_K4642028 and BBa_K4642014 are multiplexed allowing us to detect 4 diseases at once. Each switch has their own binding site and reporter protein depending on the condition the switch is designed to detect. This composite part is a recreation of this circuit without the associated anti-miRNA of the switches.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 973
    Illegal BsaI.rc site found at 1017
    Illegal SapI.rc site found at 2939


[edit]
Categories
Parameters
None