Composite

Part:BBa_K4642031

Designed by: Ryan Yapa   Group: iGEM23_City-of-London-UK   (2023-09-28)
Revision as of 14:56, 11 October 2023 by RishabhDas (Talk | contribs)

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T7 / Gen4 320b-320a-4640 Double AND-Gate / mCerulean / Double Terminator

This composite part is used for testing the presence of hsa-miR-320b, hsa-miR-320a and hsa-miR-4640. It has a T7 promoter for expression of functional RNA BBa_K1614000. This is followed by the code for our toehold switch BBa_K4642028. Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is mCerulean BBa_K3075004. The fluorescence produced can be detected with a luminometer to affirm a positive test.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None