Composite

Part:BBa_K4642020

Designed by: Ryan Yapa   Group: iGEM23_City-of-London-UK   (2023-09-28)
Revision as of 14:55, 11 October 2023 by RishabhDas (Talk | contribs)

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T7 / Gen4 486-19b-1260a Double AND-Gate / GFP / Double Terminator / T7 / Gen4 486-19b-1260a Double A

This composite part is used for testing the presence of hsa-miR-486, hsa-miR-19b and hsa-miR-1260a. It has a T7 promoter for expression of functional RNA BBa_K1614000. This is followed by the code for our AND-gate toehold switch BBa_K4642014 and the anti-miRNAs BBa_K4642015 and BBa_K4642016. Placing a reporter protein downstream of this toehold switch part will mean that when our trigger RNAs are present in high enough quantities, the protein in the CDS will be translated and the reporter protein can be observed. Our chosen reporter protein is GFP, BBa_I746916. This fluorescence produced can be detected with a luminometer to affirm a positive test.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 178


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Categories
Parameters
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