Coding
Wza-SC

Part:BBa_K4614112

Designed by: Yao-han Huang   Group: iGEM23_CAU-China   (2023-10-11)
Revision as of 14:51, 11 October 2023 by Registry (Talk | contribs)

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Encoding sequence of the fusion protein of Wza and SpyCatcher

Wza is an integral outer membrane lipoprotein, which is essential for group 1 capsule export in Escherichia coli. The transmembrane region is a novel α-helical barrel. Mature Wza, the best studied OMA member, is a 359-residue lipoprotein protein that forms SDS-stable octamers. The C terminus of each monomer is exposed on the cell surface, placing the acylated N terminus at the inner leaflet of the outer membrane, consistent with typical outer membrane lipoproteins[1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 661


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Categories
Parameters
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