Coding

Part:BBa_K4825017

Designed by: Ye Siyi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 14:50, 11 October 2023 by Registry (Talk | contribs)

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ATF1

ATF1 is derived from S.cerevisiae genome. It encodes for the enzyme alcohol acetyltransferase 1 (AATase 1), and catalyses the reaction of acetate esters from acetyl-CoA and alcohols. It is included in our composite part with ARO10 (2-ketoacid decarboxylase) and ADH7 (alcohol dehydrogenase), which are transformed into the genome of S.cerevisiae simultaneously for overexpression to produce isoamyl acetate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 412
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 412
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 412
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 412
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 412
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534


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Categories
Parameters
None