Composite

Part:BBa_K4880020:Design

Designed by: Xinran Xia   Group: iGEM23_BASIS-China   (2023-09-08)
Revision as of 14:15, 11 October 2023 by Vivixia (Talk | contribs)


PpsbA2-sepA1-PisiA6803-sepT1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 490
    Illegal PstI site found at 1438
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1438
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 691
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 490
    Illegal PstI site found at 1438
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 490
    Illegal PstI site found at 1438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 945
    Illegal SapI site found at 502
    Illegal SapI.rc site found at 476


Design Notes

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Source

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References

Zhou, Y., Sun, T., Chen, Z., Song, X., Chen, L., & Zhang, W. (2019). Development of a new biocontainment strategy in model cyanobacterium synechococcus strains. ACS Synthetic Biology, 8(11), 2576–2584. https://doi.org/10.1021/acssynbio.9b00282